top of page
  • Writer's pictureEJPPS

Updated: Mar 30, 2022

Peer Review Article | Open Access | Published 25th March 2022


A review on analytical methods of cilnidipine and its combinations


Pranali Mishra, Ankit Mishra, Parul D Mehta | EJPPS | 271 (2022) | https://doi.org/10.37521/ejpps.271011 Cite this article | Click to download pdf



Abstract


Background - The chase to improve the quality of life has stimulated desirable changes in research to design and develop a new drug and enhance its safety and effectiveness. Thus, there is a gradual rise in demand to develop susceptible and specific analytical techniques for newly developed drugs. Thus, analysts are striving very hard to develop new and efficient analytical methods to achieve these targets.


Main body of abstract - Analytical methods that analyze drug compounds in a given matrix need to be optimized and validated to ensure excellent selectivity, sensitivity, ease of use, speed of analysis, less expensive, and efficient analytical procedures. Developing a new analytical method should be considered critical, based on availability and accurate handling of different instruments. This review is a genuine venture of compiled literature of earlier and recent trends in the method developments for Cilnidipine (CLD) analysis alone and in combination with other drugs. It provides an in-depth assortment of practical aspects of various analytical techniques published for CLD.


Conclusion - High-performance liquid chromatography and ultraviolet spectroscopy have been found the most acceptable for the analysis of CLD. Stability indicating studies and impurity profiling of CLD also prevailed in the assembled literature. Scanty work was observed with capillary electrophoresis, Fourier transform-infrared spectroscopy, and electroanalytical methods to analyze CLD. Applications mentioned for CLD are significant in their particular field and contribute to analytical assay in future endeavours.

Keywords- Cilnidipine, Bioanalytical method, Stability indicating method, HPLC, Spectrophotometry.

Introduction


Hypertension is one of the most significant observed public health challenges contributing to cardiac disease and death globally. Hence, to lower the risk for cardiovascular disease, it is admissible to control blood pressure strictly. Combining two or more antihypertensive agents with different action mechanisms can be used reliably to achieve the aforementioned condition. This combination therapy has proved beneficial in preventing major cardiovascular diseases, reducing the risk for adverse effects, and maximizing drug compliance ¹ ².


Calcium channel blockers (CCB) are first-line drugs in the treatment of hypertension. However, CCB alone was insufficient in lowering blood pressure. Hence, CCBs have been widely co-administered to treat hypertension. These drugs act by inhibiting calcium (Ca)-channels in the myocardium and vascular smooth muscle cells, which lowers the myocardial contractions, decrease pulse conduction, and causes vasodilation. Thus, they are found to be effective in the treatment of essential hypertension. Furthermore, among the three main classes of CCBs, 1,4-dihydropyridines (DHP) have contributed to a widely used hypotensive drug class ³ ⁴.


Among various 1,4-dihydropyridine CCBs, Cilnidipine (CLD) shows unique action on sympathetic N-type Ca-channels, besides acting on L-type Ca-channels, as with most Ca-channel antagonists. Their action is performed through vasodilatation, decreased heart rate, and increased renal blood flow ⁵. CLD has opted as CCB of choice in hypertensive patients with diabetes, chronic kidney disease, and patients developing edema. It is a novel 4th generation CCB ⁶. It is found to dilate both efferent and afferent arterioles resulting in decrease in pressure in the capillary bed. Hence, the accumulated fluid of tissues flows back to veins, thus skipping pedal edema incidence ⁷. It shows a slow onset but long-lasting hypotensive effect by inhibiting sympathetic neurotransmission and norepinephrine release. It shows excellent selectivity for vascular smooth muscle. CLD has also emerged as a good candidate for combination therapy ⁸ ⁹.


CLD is chemically described [Figure 1] as 2-Methoxyethyl (2E)-3-Phenyl-2-propen-1-yl-2,6-dimethyl-4-(3-nitrophenyl)-1,4-dihydro-3,5-pyridinedicarbox¬ylate ¹⁰ ¹¹. The development of CLD can be credited jointly to Fuji Viscera Pharmaceutical Company, Japan, and Ajinomoto, Japan which was approved in 1995. Countries like China, Japan, Korea, India, and several countries in the European Union have approved this drug ¹². CLD is a light yellow-coloured crystalline powder. It is insoluble in water. The molecular weight of CLD is 492.528 g/mol, and the molecular formula is C27H28N2O7. Absorption of CLD is rapid; maximum peak concentration is achieved in 2 hours. Distribution in kidneys, liver, plasma and other tissues is high. Even after repeated oral administration, accumulation of CLD is not observed. It has a large volume of distribution. It shows low bioavailability due to low aqueous solubility and high permeability ¹³. Microsomal enzymes highly metabolize it with a dehydrogenation process in both the liver and kidney. Elimination is 20% through the urine and 80% through feces. The half-life of the hypotensive effect for CLD is about 20.4 min. It shows cardioprotective, renoprotective, and neuroprotective effects. Administration of CLD has been shown to decrease blood pressure safely and effectively, without excessive blood pressure reduction or tachycardia ¹⁴.


Methods of analysis –

Various analytical methods for the determination of CLD are presented here as compendial and reported methods. Compendial methods are official methods.


Compendial method

Japanese Pharmacopeia and Indian Pharmacopeia approved CLD in 2018 in 2016, respectively. The identification method for CLD includes Ultraviolet (UV)/ Visible (Vis)-spectrophotometry and infrared spectrophotometry. In the Japanese Pharmacopeia, chromatographic separation was achieved on stainless steel column with dimensions 25 cm x 4.6 mm and particle size of 5 μm, and the mobile phase reported is a mixture of sodium acetate buffer and methanol. The detection wavelength is 240 nm using a UV detector. The column temperature mentioned is 25∘C ¹⁵.


The Indian Pharmacopeia also mentioned chromatographic separation using Phenomenex-Prodigy ODS 3V column of dimension 25 cm x 4.6 mm and particle size of 5 μm. The mobile phase reported is a mixture of acetonitrile (ACN): 0.01M sodium acetate buffer (70:30% v/v). The flow rate is reported to be 1.0 mL/min. The injection volume is 20 μL, and the detection wavelength was 240 nm using a UV detector ¹⁶.


Reported methods of analysis

The rapid progress of science and technology has led to the development of numerous newly synthetic drugs prompting the development of analytical methods for determining these drugs in the manufacturing phase of the pharmaceutical formulations and their determination in the human body. Thus, the analysis of pharmaceuticals has gained progressive importance in the overall drug development process. This study aimed to comprehensively review the literature and collect the evidence concerning the analysis of CLD and its combinations of dosage forms. Data was assembled by search on Google Scholar, Pubmed, and Elsevier’s Science Direct. The keywords included “estimation of cilnidipine”, “analytical method development of cilnidipine,” “pharmaceutical preparations of cilnidipine,” “cilnidipine in biological fluids.” Figure 2 provides chronological reported methods for estimation of CLD. The results show that CLD can be estimated by spectrophotometry, High- performance liquid chromatography (HPLC), Liquid chromatography-mass spectroscopy (LC-MS), High- performance thin layer chromatography (HPTLC), voltammetry, capillary electrophoresis, and spectrofluorimetry, either in the form of raw materials or pharmaceutical preparations. Different analytical methods for estimating CLD as reported in the literature are comparatively provided in Figure 3. This review provides a complete insight for the analysis of CLD, alone or in combination in pharmaceutical preparations or biological fluids. Figure 4 provides comparative data of estimation methods for CLD alone and in combination with other drugs.


Spectrophotometry methods

Spectrophotometry is concerned with the quantitative measurement of a material’s reflecting or transmitting properties as its function of wavelength. It represents several advantages: simple, fast, easy to perform, less expensive, and accurate for the routine analysis of an analyte in bulk or pharmaceutical preparations in a quality control laboratory ¹⁷. Literature reveals numerous methods for the analysis of CLD through UV/Vis-spectrophotometry ¹⁸⁻⁵⁴. Methanol was mainly used as a choice of solvent for analysis. Various spectrophotometric methods employed were simultaneous equation method, absorbance ratio method, first and second derivative spectroscopic method, Vierodt’s method, and dual-wavelength method. UV/Vis-spectrophotometric method reported for estimating CLD as alone or combined with other drugs is represented in Table 1.


In a special method, El Hamd MA and his coworkers developed an indirect method, using N-bromo succinimide (NBS) and indigo carmine (INC) dye to quantify five different drugs of the 1,4-DHP category ⁵⁴. They followed a fascinating mechanism, wherein they added the acidic solution of 1,4-DHP drugs with an excess amount of NBS. They calculated the excess amount of unreacted NBS bleached with the dye from the drug corresponding to reacted NBS. They reported obeying the Beer-Lambert law in the concentration range of 1.25–13.00 μg/mL. They found excellent correlation coefficient values and percent recoveries. Detections limits ranged between 0.141 to 0.5 μg/mL. The method found successful application for the analysis of different dosage forms.


Liquid chromatography

Chromatography refers to the separation of analyte as an individual component from a mixture and assessing it thoroughly. It is the most extensively used technique in pharmaceutical analysis. Among chromatographical techniques, special attention is gained by HPLC and emerging ultra-performance liquid chromatography (UPLC), which mainly enhance “speed, resolution, and sensitivity” to separate, identify, and quantify each component in a mixture ⁵⁵ ⁵⁶. In reported methods, ²⁰ ⁴⁶ ⁵⁷⁻⁹⁰] separation is mainly achieved on the C18 column, and the mobile phase consists mainly of ACN and a buffer of potassium phosphate, pH ranges between 3-5. The flow rate observed mainly was 1 mL/min. The chromatography method reported for estimating CLD as alone or in combination with other drugs, i.e., Telmisartan (TELMI), Olmesartan (OLM), Metoprolol succinate (METO), Amlodipine mesylate (AMLO), Bisoprolol fumarate (BF), Chlorthalidone (CHL), Nebivolol HCl (NEBI), Valsartan (VAL), Atenolol (ATEN) and Irbesartan (IRB) is represented in Table 2.


Analytical eco-scale and quality by design-oriented liquid chromatography

In a single reported method, Panda SS et al. developed an RP- HPLC method to estimate CLD, TELMI, and METO, employing an eco-scale and quality-by-design approach ⁹⁰. They separated drugs on the C18 column using a mobile phase consisting of a mixture of methanol and 0.01 M KH2PO4 buffer of pH 3.0 at a ratio of 70:30% v/v with a flow rate of 1.0 mL/min. They quantified the drugs with a diode array detector at 240 nm. They assessed method variables using the Box-Behnken design. Well-separated peaks, with a resolution greater than 2.0, of all the three analytes were obtained. They observed linearity between 2.5-80 μg/mL, accuracy was >99%, and precision was <1%. The eco-scale analysis showed an excellent green score for the present all three drugs. The optimized method quantified the analytes from pharmaceutical dosage forms and showed excellent and acceptable results for a limit of detection (LOD), the limit of quantitation (LOQ), system suitability, and solution stability. The green analytical method can be successfully applied for simultaneous estimation of combined pharmaceutical formulations.


Stability indicating estimation of CLD

The stability-indicating assay method is a validated quantitative analytical method that detects chemical, physical or microbiological change with time. These assays generally include forced degradation/stress testing to produce specific drug products in which active ingredients and degradation products can be measured accurately without interference. The HPLC method is widely used as a research tool in analytical techniques to estimate degradation impurities in drug substances and products. Common stress-induced conditions include acid, base, oxidative, photolytic, thermal, and humidity stresses ⁹¹ ⁹².


A thorough literature search revealed that various assay procedures such as RP-HPLC, HPTLC, and LC/MS/MS are available for the stability study of CLD ⁷² ⁹³⁻¹¹⁰. In all the studies reviewed, separation was achieved on a C18 column, and the mobile phase consisted mainly of ACN, methanol, and buffers such as potassium monophosphate, orthophosphoric acid and acetate buffers. The flow rate was between 0.8-1.2 mL/min. Generally, the detectors used were photodiode array (PDA) detectors and mass spectrometry (MS). Estimations were carried out at room temperature. The stability-indicating method was reported to estimate CLD as alone or in combination with other drugs like METO, TELMI, VAL, NEBI, OLM, CHL, and IRB and the results are represented in Table 3.


In a special method, Ch KR et al. worked with liquid chromatography coupled with quadrupole time of flight mass spectrometry (HPLC-QTO/MS). They developed an analytical method to study stress degradation studies of seven degradation products as per the International Conference on Harmonization (ICH) Q1A (R2) guidelines, characterized using high-resolution mass spectrometry ¹¹⁰. Three oxidative degradation products, CD1, CD2, and CD3, were separated from the analyte’s mixture with the HPLC C18 column. Structure elucidation was performed by a high-resolution mass spectrometer, nuclear magnetic resonance, and spectroscopic techniques. Degradation showed the formation of racemic mixtures for two degradative products, which chiral HPLC verified. They used ROESY data for fixing stereocenters for CD1 and its enantiomer and CD2 and its enantiomer. They observed novel structures of CD1 and CD2.


Stability indicating estimation of CLD with UPLC

UPLC provides better separation capabilities than HPLC with added benefits such as increased resolution, sensitivity, and speed of analysis with shorter run time and lower solvent consumption. Due to its benefits, this technique has gained considerable attention to analyze pharmaceutical and biomedical compounds of interest ¹¹¹. Only two methods were reported in the literature under stability-indicating estimation of CLD with UPLC.


Alagar RM et al. have developed and validated the UPLC method as per the ICH/FDA regulatory requirements for estimating CLD and OLM in its pharmaceutical dosage form ¹¹². Chromatographic separation using BEH C18 column (100 × 2.1 mm, 1.7 μm), and pH 3.5 buffer: methanol in ratio 35:65% v/v as mobile phase with a flow rate of 0.3 mL/min was achieved. The detection wavelength was selected to be 254 nm. Different stress conditions for CLD and OLM implemented were thermal stress, photolytic stress, acid media, alkali media, and oxidative media. Retention time values obtained for degradation products were significantly different from those of pure drugs. Linearity was found in the concentration range of 0.2-0.3 μg/mL. Good precision and smaller relative standard deviations were seen. Analyte recovery was acceptably between 99.04-101.58%. The proposed method can find application in quality control industry laboratories for various pharmaceutical dosage forms.


Shah PK et al. have developed a validated stability-indicating RP-HPLC method to estimate OLM medoxomil, CHL, and CLD and their impurities ⁷². Analytes were separated on Hypersil-BDS Thermo-Scientific, C18 (12.5 cm x 4.6 mm, 5μm) using mobile phase ammonium acetate solution (pH 5): ACN in gradient mode at 25C. The flow rate was 1mL/min, and the detection wavelength was 260 nm. CHL impurity and OLM impurity showed retention at 2.7 and 7.2 minutes, respectively. The retention time of OLM was found at 3.3 min. The stress degradation products showed acceptable separation from analytes and impurities. The developed method was accurate, sensitive, specific, linear, and with less run-time. Hence it can be successfully applied for routine quality control and stability analysis of pharmaceutical dosage form.


Bioanalytical methods

Bioanalytical methods are the quantitative assessment of the concentration of the drug, its metabolite, and biomarker in biological fluids, such as blood, plasma, serum, urine, saliva, or tissue extracts. This method is characterized by speedy analysis, sensitivity, and robustness, which are imperative to fulfill the validation guidelines requirements, including accuracy, precision, sensitivity, selectivity, reproducibility, and stability ¹¹³. These methods enable the separation, identification, and determination of many biologically active compounds ¹¹⁴. The literature revealed many reliable bioanalytical methods, which can be applied successfully and conveniently for the intended research purpose ¹¹⁵⁻¹²¹. It was observed that in most bioanalytical methods, sample preparation was performed using protein precipitation (PPT), liquid-liquid extraction (LLE), or solid-phase extraction (SPE) method. Separation was mainly achieved on the C18 column, and the mobile phase consisted mainly of ACN, methanol, and buffers. Commonly used detectors were PAD, MS, and fluorometric. The bioanalytical method reported for estimating CLD as alone or in combination with other drugs like VAL, CHL, NEBI, and AMLO is represented in Table 4.


HPTLC Methods

HPTLC is the separation and identification method having universal expectance for various chemical and biological mixtures ¹²². This method uses various solvents as mobile phases and a variety of solids as stationary phases. In HPTLC, mobile phases can be used in various manners, such as isocratic or mixed solvents. The addition of various modifiers can alter the nature of mobile phases. HPTLC offers several advantages, including better resolution, lesser sample size, smaller volumes of mobile phases, etc. The literature survey on HPTLC of CLD with other drugs has been summarized in Table 5 ¹²³⁻¹³².


Estimation of CLD with impurities

Impurity profiling of drugs in pharmaceutical research is the process of acquiring and evaluating data that establishes the biological safety of an individual impurity as per the guidelines published by the ICH. It is designed to detect, identify or elucidate the structure and quantify organic and inorganic impurities in bulk drugs and pharmaceutical formulations. Few methods are found through literature surveys that reported various impurities present in CLD ¹³³ ¹³⁴. Subsequent methods are summarized here.


Kasimala BB et al. developed a validated stability-indicating RP- HPLC method to quantify CLD and its related impurities in pharmaceutical formulations ¹³⁵. The column employed for separation was an X Terra (250 × 4.6 mm; 5 μm) C18 column. The mobile phase used was methanol and phosphate buffer of pH-5.8 (10:90% v/v) with isocratic elution at a 1.0 mL/min flow rate. UV detector at 229 nm wavelength was used to detect the eluents. Linearity was found to be in the range of 2-12 μg/mL. The recovery rate was more than 98% for each analyte. They found stress degradation studies showed that UV light exposure degraded the analyte up to 9.967% and base hydrolysis showed the analyte degradation up to 6.223%. Conditions like acidic stress (5.347%), oxidative stress (4.916%), and thermal stress (4.319%) conditions showed no interference of both known impurities with CLD. The developed method was precise, robust, selective, specific, and suitable for quantifying and determining CLD impurities in pharmaceutical formulations.


Masada S et al. developed an HPLC method to detect N-nitroso dimethylamine (NDMA) contaminated VAL and CLD tablets ¹³⁶. They initially assayed a standard solution for the quantitative estimation, followed by quantitative estimation in gradient elution mode with mobile phase water-ACN and 0.1% formic acid to detect components simultaneously. The retention times of NDMA, VAL, and CLD were acceptably separated at 7.8, 16.3, and 17.1 min, respectively. They found linearity in the range of 0.0111–7.4 μg/mL and a correlation coefficient of 0.999. The LODs and LOQs were 0.0085 μg/mL and 0.0285 μg/mL, respectively. They also reported a GC-MS method for detecting NDMA in VAL & CLD combination, successfully with much lower LOD. The method used was in the isotopic mode and required multiple extraction steps. They concluded that the developed HPLC method was low-cost and more suitable for rapidly screening NDMA contaminated VAL CLD combination with sufficient sensitivity.

Zeng H et al. developed a liquid chromatography/Q-Orbitrap mass spectrometry (LC/Q-Orbitrap MS) method to investigate the structural information photodegradation impurities of CLD ¹³⁷. Boston Group C18 column (250 × 4.6 mm, 5μm) was employed for chromatographic separation, whereas ACN: H2O in the ratio of 75:25% v/v was used as mobile phase. Detection was carried out using a Thermo LC system coupled with a Q-Orbitrap high-resolution mass spectrometer with both positive and negative ion modes. To carry out a systematic forced degradation study, CLD underwent photolysis. The structural information of the detected five photodegraded impurities of cilnidipine was determined by LC/MS/MS analysis. Structure elucidation was performed with 1H-NMR and 13C-NMR data. Two photodegradation pathways to produce different photodegradation impurities were also revealed in the study. The optimized method provided good results and can be successfully applied to investigate separated and characterized drug substances.


Hu CC & Gu X developed an HPLC-QTO/MS method to identify the light degradation impurity of CLD ¹³⁸. Chromatographic separation was performed on the C18 column (250 × 4.6 mm, 5μm). The impurity was initially synthesized, and then the structure was identified by the full scan with NMR. Comparative analysis of UV spectra, the mass spectra, and retention time of this impurity of synthesized impurity was performed. The chemical structure of light degradation impurity was a Z-isomer of CLD in tablet and capsule. The method provides valuable scientific data for studying the preparations, the photodegradation behaviour, and the quality control of degradation impurities of CLD.

Wang SG & Gu P developed an RP-HPLC method to estimate CLD related substances using a C18 chemical column at 40∘C column temperature ¹³⁹. The mobile phase employed was ACN and 0.025 mol/L ammonium dihydrogen phosphate solution and cyclohexane ratio 60:39:1 at a 1.5 mL/min flow rate. Detection was accomplished at 240 nm with a UV detector. The drug peak and impurity peak were well separated. Linearity was found in the range of 1-16 mg/L. RSD was found as 0.42%. The developed method showed suitability and sensitivity for the determination of CLD related substances.


Zeng H et al. performed an impurity profiling study of CLD tablets and capsules by HPLC-QTO/MS and evaluation of packaging materials ¹⁴⁰. The source of the impurities was investigated. HPLC was used to analyze impurities produced on photodegradation; the method showed significant results for separating and identifying impurities in cilnidipine tablets and capsules. They compared four different pharmaceutical packaging materials for impurities. Impurity II was produced by direct exposure to light, and impurity III was produced from the ethanol solution of CLD. UV-vis spectrophotometer detected the shading effect of the four packaging materials, which showed a remarkable difference in the formation of photodegradation products. HPLC-QTO/MS/MS elucidated the structure of five impurities in commercial cilnidipine tablets and capsules. High-resolution MS/MS data successfully separated and identified the impurities. Thus, the method can find application for the successful identification of impurities in CLD in pharmaceutical preparations. An animal study was also performed with the impurities and found that impurity II and impurity III were cytotoxic. Based on the result, methods suggested modification of packaging material for CLD tablets.


Capillary electrophoresis and enantio-separation of cilnidipine

Du Y & Di B developed a rapid and straightforward capillary electrophoresis method with high separation efficiency using a synthetic polysaccharide, desulphated chondroitin sulfate C, as a novel chiral selector ¹⁴¹. It was applied to the enantiomer separation of the racemic mixture of CLD. The chiral separation was investigated for studying the effects of concentration of pH, buffer, and applied voltage. The optimum resolution conditions showed pH 2.50, concentration to be 30 g/L, and voltage applied to be 10 kV. The successfully resolved CLD enantiomers showed a good resolution factor of 2.01.


Supercritical Fluid chromatography (SFC)

Zhang L et al. developed a rapid, environmentally friendly, and potential SFC coupled with two-phase hollow fibre-based liquid-phase microextraction for chiral separation of seven most commonly used 1,4-DHP ¹⁴². Separation was achieved on immobilized polysaccharide chiral selectors coated with cellulose-tris (3,5-dichlorophenylcarbamate). Isopropanol was used to modify resolution; as such maximum resolution of 13.38 was obtained. Within optimal conditions, nimodipine enantiomers showed the LOD as 0.3 and 0.5 μg cm−3. A total of 80.0–99.8% recoveries were observed. Additionally, the developed SFC technique was found to be an effective and environment-friendly method that can successfully find its application for separating and quantifying 1,4-DHP enantiomers and other pharmaceutical drugs.


Fourier transform infrared (FT-IR)

One of the reliable analytical methods is infrared (IR) spectroscopy which provides rapid, label-free, and objective analysis for the active pharmaceutical ingredients in the pharmaceutical industry. It is more considered as an attractive and promising analytical tool regarding process analytical technology and green chemistry. IR spectroscopy has gained wide industrial acceptance for routine analysis ¹⁴³.


Literature survey reveals that Patel A et al. have developed and validated the FT-IR spectrometric analytical method for quantitative estimation of a single dosage of CLD in tablet form ¹⁴⁴. Absorbance was measured for carbonyl group (C=O) peak at 1697 cm-1 and plotted against concentration to obtain a calibration curve and calculate all the regression parameters. Linearity was obtained by overlaying the spectra of all individual concentrations. For CLD calibration curve was plotted over a concentration range of 5-25 μg/mg. Recoveries were found to be 99.8-102.5, and 99.8- 101.4% RSD was observed to be less than 2. The detection limit was 0.22 and 0.050 μg/mg; the LOQ was 0.60 and 0.17 μg/mg. The developed method suggested that FT-IR is a simple, rapid, reproducible, and less time-consuming analytical method that can find its application for the direct determination of CLD in pharmaceutical formulations.


Voltametric Detection of Calcium Antagonist Cilnidipine

Jain R has developed a susceptible and selective sensor voltammetric detection method to estimate CLD ¹⁴⁵. They characterize the fabricated sensor by cyclic voltammetry, chrono-coulometry, electrochemical impedance spectroscopy, square wave voltammetry, and scanning electron microscopy. They enhanced the sensor’s sensitivity by fabricating hybrid film by increasing the surface area and active electron transfer sites. Thus, it showed high sensitivity and selectivity in performance as compared to bare glassy carbon, zinc oxide nanoparticle modified, and multi-walled carbon nanotubes modified electrodes. The linearity of CLD was found to be in the range of 5 ng/mL to 5 μg/mL. The proposed electrochemical method was helpful in the electroanalytical determination of CLD in its pharmaceutical formulation and will find application for the routine analysis of other pharmaceutical formulations.


Fluorescence probe for CLD assay

Tan S et al. carried out a fluorescence assay for CLD. A fluorescence probe was encapsulated 1-naphthaleneboronic acid (NBA), with an emulsion polymerization technique, using Triton-X 100, EDMA, butyl methacrylate, and potassium persulfate [146]. The average diameter was 764.1 nm for the resultant polymer particles in the phosphate buffer solution. It showed high sensitivity and was used for CLD assay based on fluorescence quenching. Linearity of CLD was observed over the concentration range of 2.0×10⁻⁷ to 1.1×10⁻⁵ mols/L. The NBA-encapsulated probe showed a significantly improved response to that of free NBA. The proposed method can find application for analysis of other calcium blockers in bulk and pharmaceutical formulations.


Conclusions

An insight of the present work comprehensively serves as a systematic review of the current analytical methods for determining CLD and its combination in pharmaceutical and biological samples like serum and plasma. The past and present scenario of widely accepted analytical methods for analyzing CLD has been systematically presented. These methods include spectrophotometry, spectrofluorimetry, liquid chromatography, electroanalytical methods, and FT-IR. Figure 5 provides distinguished estimation methods for various combinations of CLD. From the literature, it is observed that due to its advantages such as sensitivity, specificity, and better separation efficiency, over other analytical techniques, HPLC has been found as the most acceptable application for the analysis of CLD. It has been observed that other analytical methods too showed prominent utilization for its determination. Although there are numerous well-defined and validated methods for the quantification analysis of CLD, these methods lack the principles of “green chemistry.” Henceforth, there is a rise in demand to develop eco-friendly methods that will diminish toxic organic effluents, hazardous to the environment. The presented systematic information is a rapidly developing subject that can be anticipated for its utility in the near future to researchers involved in formulation development and quality control of CLD.


List of Abbreviations

1,4-dihydropyridines

DHP

​1-Naphthaleneboronic acid

NBA

Acetonitrile

ACN

Amlodipine mesylate

AMLO

Ammonium acetate buffer

NH₄Ac

Atenolol

ATEN

Azilsartan

AZT

Bisoprolol fumarate

BF

Calcium

Ca

Calcium channel blockers

CCB

Centimeter

cm

Chloroform

​CHCl₃

Chlorthalidone

​CHL

​Cilnidipine

CLD

​Ethanol

EtOH

Fourier transform infrared

FT-IR

Glacial acetic acid

GAA

High-performance liquid chromatography

HPLC

Indigo carmine

INC

International Conference on Harmonization

ICH

​Irbesartan

IRB

​Limit of detection

LOD

​Limit of quantitation

LOQ

​Liquid chromatography coupled with quadrupole time of flight mass spectrometry

HPLC-QTO/MS

Liquid chromatography-mass spectroscopy

LC-MS

​Liquid-liquid extraction

LLE

Methanol

MeOH

Metoprolol succinate

METO

Microgram/Milliliter

​μg/mL

Millimeter

mm

Nanometer

nm

​N-Bromo succinimide

NBS

Nebivolol HCl

​NEBI

Nifedipine

​NIF

N-nitroso dimethylamine

​NDMA

Normal phase

NP

Olmesartan medoxomil

​OLM

Orthophosphoric acid

​OPA

​Performed using protein precipitation

​PPT

​Photodiode array

PDA

Potassium dihydrogen orthophosphate buffer

​KH₂PO₄

Reverse phase

​RP

Sodium hydrogen phosphate

​Na₂HPO4

Sodium hydroxide

​NaOH

Solid-phase extraction

SPE

Telmisartan

TELMI

​Triethylacetic acid

TEA

Ultra violet

UV

Ultra-performance liquid chromatography

UPLC

​Valsartan

VAL

Visible

Volume/Volume

v/v

Table1 Spectrophotometric specifications and results for determination of CLD alone and with other drugs in the bulk drugs and pharmaceutical dosage forms


CLD-Cilnidipine; BF-Bisoprolol Fumarate; TELMI-Telmisartan; OLM-Olmesartan medoxomil; METO-Metoprolol succinate; BF-Bisoprolol; CHL-Chlorthalidone; NEBI-Nebivolol HCl; VAL-Valsartan; AZT-Azilsartan; ACN-Acetonitrile; EtOH-Ethanol; MeOH-Methanol; LOD-Limit of detection; LOQ-Limit of quantitation. NH4Ac-Ammonium acetate buffer; --Not provided.


Table 2 Chromatographic specifications and analytical specifications of HPLC methods for determination of CLD alone and with other drugs in the bulk and pharmaceutical dosage forms

CLD-Cilnidipine; TELMI-Telmisartan; OLM-Olmesartan medoxomil; METO-Metoprolol succinate; AMLO-Amlodipine mesylate; BF-Bisoprolol fumarate; CHL-Chlorthalidone; NEBI-Nebivolol HCl; VAL-Valsartan; ATEN-Atenolol; IRB-Irbesartan; UV-Ultra violet; ACN-Acetonitrile; EtOH-Ethanol; MeOH-Methanol; KH2PO4-Potassium dihydrogen orthophosphate buffer; TEA-Triethylacetic acid; OPA-Orthophosphoric acid; NaOH-Sodium hydroxide; PDA-Photodiode array; LOD-Limit of detection; LOQ-Limit of quantitation; NH4Ac-Ammonium acetate buffer; --Not provided.


Table 3 Chromatographic specifications and results of Stability indicating studies method for determination of CLD alone and with other drugs in the bulk drugs and pharmaceutical dosage forms

CLD-Cilnidipine; TELMI-Telmisartan; OLM-Olmesartan medoxomil; METO-Metoprolol succinate; CHL-Chlorthalidone; NEBI-Nebivolol HCl; VAL-Valsartan; ATEN-Atenolol; IRB-Irbesartan; UV-Ultra violet; ACN-Acetonitrile; EtOH-Ethanol; MeOH-Methanol; KH2PO4-Potassium dihydrogen orthophosphate buffer; OPA-Orthophosphoric acid; Na2HPO4-Sodium hydrogen phosphate; NaOH-Sodium hydroxide; PDA-Photodiode array; LOD-Limit of detection; LOQ-Limit of quantitation; NH4Ac-Ammonium acetate buffer; GAA- Glacial acetic acid; --Not provided.


Table 4 Chromatographic specifications and results of Bioanalytical method for determination of CLD alone and with other drugs in the bulk drugs and pharmaceutical dosage forms

CLD-Cilnidipine; CHL-Chlorthalidone; NEBI-Nebivolol HCl; VAL-Valsartan; AZT-Azilsartan; NIF- Nifedipine; NIMO-Nimodipine; AMLO-Amlodipine; UV-Ultra violet; ACN-Acetonitrile; EtOH-Ethanol; MeOH-Methanol; KH2PO4-Potassium dihydrogen orthophosphate buffer; OPA-Orthophosphoric acid; Na2HPO4-Sodium hydrogen phosphate; NaOH-Sodium hydroxide; PDA-Photodiode array; LOD-Limit of detection; LOQ-Limit of quantitation; NH4Ac-Ammonium acetate buffer; GAA- Glacial acetic acid; MS-Mass spectrophotometer; ESI-Electron spray ionization; LLE-Liquid liquid extraction; PPT- protein precipitation technique; methyl-t-butyl ether (MTBE)


Table 5 Chromatographic specifications and results of HPTLC method for determination of CLD alone and with other drugs in the bulk drugs and pharmaceutical dosage forms

NP-Normal phase; RP-Reverse phase; HPTLC: High-Performance Thin Layer Chromatography; CHCl3-Chloroform; MeOH-Methanol; NIF- Nifedipine; CLD-Cilnidipine; TELMI-Telmisartan; OLM-Olmesartan medoxomil; METO-Metoprolol succinate; AMLO-Amlodipine mesylate; BF-Bisoprolol fumarate; CHL-Chlorthalidone; NEBI-Nebivolol HCl; VAL-Valsartan; AZT-Azilsartan; ATEN-Atenolol; IRB-Irbesartan; UV-Ultra violet; ACN-Acetonitrile; EtOH-Ethanol; MeOH-Methanol; KH2PO4-Potassium dihydrogen orthophosphate buffer; TEA-Triethylacetic acid; OPA-Orthophosphoric acid; Na2HPO4-Sodium hydrogen phosphate; NaOH-Sodium hydroxide; PDA-Photodiode array; LOD-Limit of detection; LOQ-Limit of quantitation; NH4Ac-Ammonium acetate buffer; GAA- Glacial acetic acid.


 

References


01. Harada E, Sugino K, Aimoto M, Takahara A. Effects of the L/N-Type Ca2+ Channel blocker cilnidipine on the cardiac histological remodelling and inducibility of atrial fibrillation in high-salt-fed rats. Biological and Pharmaceutical Bulletin 2021; 44(5): 707-713. Doi:10.1248/bpb.b21-00024.

 

02. Yamashita T, Kamikaseda S, Tanaka A, Tozaki-Saitoh H, Caaveiro JM, Inoue K, Tsuda M. New inhibitory effects of cilnidipine on microglial P2X7 Receptors and IL-1β Release: An Involvement in its Alleviating Effect on Neuropathic Pain. Cells 2016; 10(2): 434. Doi: 10.3390/cells10020434.

 

03. Shete MM. Cilnidipine: next generation calcium channel blocker. J Assoc Physicians India 2016; 64(4): 95-99.

 

04. Godfraind T. Discovery and development of calcium channel blockers. Front Pharmacol 2017; 8:286.

 

05. Yoshimoto R, Dohmoto H, Yamada K, Goto A. Prolonged inhibition of vascular contraction and calcium influx by the novel 1, 4-dihydropyridine calcium antagonist cinaldipine (FRC-8653). The Japanese Journal of Pharmacology 1991; 56(2): 225-229.

 

06. Takahara A. Cilnidipine: a new generation Ca2+ channel blocker with inhibitory action on sympathetic neurotransmitter release. Cardiovasc Ther 2009; 27(2): 124-139. Doi:10.1111/j.1755-5922.2009.00079.x.

 

07. Takahara A, Fujita SI, Moki K, Ono Y, Koganei H, Iwayama S, Yamamoto H. Neuronal Ca2+ channel blocking action of an antihypertensive drug cilnidipine in IMR-32 human neuroblastoma cells. Hypertens Res 2003; 26(9): 743-747.

 

08. Wang AL, Iadecola C, Wang G. New generations of dihydropyridines for treatment of hypertension. J Geriatr Cardiol: JGC 2017; 14(1): 67-72.

 

09. Sweetman, Sean C. Martindale: The complete drug reference. 36th ed. Pharmaceutical Press, London. 2009; 1245.

 

10. Diwan R, Ravi PR, Pathare NS, Aggarwal V. Pharmacodynamic, pharmacokinetic and physical characterization of cilnidipine loaded solid lipid nanoparticles for oral delivery optimized using the principles of design of experiments. Colloids and Surfaces B: Biointerfaces 2020; 193: 111073.

 

11. Diwan R, Ravi PR, Agarwal SI, Aggarwal V. Cilnidipine loaded poly (ε-caprolactone) nanoparticles for enhanced oral delivery: optimization using DoE, physical characterization, pharmacokinetic, and pharmacodynamic evaluation. Pharm Dev Technol 2021; 26(3): 278-290.

 

12. Uesawa Y, Takeuchi T, Mohri K. Integrated analysis on the physicochemical properties of dihydropyridine calcium channel blockers in grapefruit juice interactions. Curr Pharm Biotechnol 2012; 13(9): 1705-1717 doi: 10.2174/138920112800958878.

 

13. Drug Profile, “Cilnidipine” https://www.chemicalbook.com/ChemicalProductroperty_EN CB2193117.htm accessed on January 2021.

 

14. Lee J, Lee H, Jang K, Lim KS, Shin D, Yu KS. Evaluation of the pharmacokinetic and pharmacodynamic drug interactions between cilnidipine and valsartan, in healthy volunteers. Drug Des Dev Ther 2014; 8:1781-1788. Doi:10.2147%2FDDDT.S68574.

 

15. Japanese Pharmacopoeia; Society of Japanese Pharmacopoeia. Tokyo, Cilnidipine 2016; 704–705.

 

16. Indian Pharmacopoeia; Indian Pharmacopoeia Commission; Ministry of Health & Family Welfare, Government of India: Ghaziabad, India, 2018, vol. 2. 1616-1618.

 

17. Kokilambigai KS, Lakshmi KS, Kumar A, Chandrayan G, Satyam K, Singh K. Spectrophotometric estimation of cilnidipine in bulk and pharmaceutical dosage form using n-(1-naphthyl) ethylene diamine dihydrochloride. Jpn J Pharmacol 1991; 56: 225-229

 

18. Fraihat SM, Al Khatib HS, Development and validation of spectrophotometric and spectrofluorimetric methods for determination of cilnidipine. Trop J Pharm Res 2020; 19(7): 1503-1509.

 

19. Firdouse S, Mohiuddin B, Begum M, Baig AA, Aquib SM. UV spectrophotometric method development and validation of Cilnidipine API and marketed pharmaceutical dosage form Int J of Pharmacy and Analytical Research 2020; 9(2): 62-67.

 

20. Sankar PR, Swathi V, Babu PS. Development and Validation of novel UV And RP-HPLC methods for determination of Cilnidipine (A New Generation Ca Channel Blocker) In Pharmaceutical Dosage Form. Int J Pharm Sci Drug Res 2019; 10(4): 1886-1894.

 

21. Jadhav RS, Ubale MB, Bharad JV. A simple, significant UV-spectroscopic analytical method development and validation for estimation of formulation drug product-cilnidipine tablet. Int J Pharm Biol Sci 2018; 8(3): 187-194.

 

22. Safhi MM. Spectrophotometric estimation of cilnidipine in tablets. J Pharm Res Int 2015; 7(6): 451-456. Doi- 10.9734/BJPR/2015/19275.

 

23. Safhi MM. Spectrophotometric method for the estimation of cilnidipine in bulk and pharmaceutical dosage forms. Ori J Chem 2013; 29(1): 131-134.

 

24. Chaudhari PP, Bhalerao AV. Method validation for spectrophotometric estimation of cilnidipine. Int J Pharm Pharm Sci 2012; 4(5): 96-98.

 

25. Pawar S, Tamboli A, Patil S. UV spectrophotometric area under curve method for the simultaneous determination of bisoprolol fumarate and cilnidipine in pharmaceutical dosage form. World J Pharm Pharm Sci 2020; 9(5): 1691-1699.

 

26. Thakare L, Ahmad S, Shastry VM. Development and Validation of UV-Visible Spectrophotometric Method for Estimation of Cilnidipine and Telmisartan in Bulk and Dosage Form. Indo Am J Pharm Res 2017; 7(04): 8552-8559.

 

27. Haripriya M, Antony N, Jayasekhar P. Development and validation of UV spectrophotometric method for the simultaneous estimation of Cilnidipine and telmisartan in tablet dosage form utilising simultaneous equation and absorbance ratio method. Int J Pharm Biol Sci 2013; 3(1): 343-348.

 

28. Vahora S, Mehta F, Chhalotiya U, Shah D. Dual Wavelength Spectrophotometric Method for Estimation of Cilnidipine and Telmisartan in Their Combined Dosage Form. Res Rev: J Pharm Sci 2014; 3(2): 22–29.

 

29. Jahan F, Jain A, Prachand S, Gupta A. Simultaneous Estimation of Telmisarta. Int J of Pharm & Research Sci 2012; 1(1): 32-42.

 

30. Sheikh F, Yeole M, Shah S, Chaple D, Ghode K, Bhongade M. Simultaneous equation spectrophotometric methods for estimation of cilnidipine and telmisartan in pharmaceutical formulation. World J Pharm Pharm Sci 2020; 9(6): 913-924. doi: 10.20959/wjpps20206-15914.

 

31. Parihar YP, Kotkar TA, Mahajan MP, Sawant SD. Development and validation of UV spectrophotometric methods for the simultaneous estimation of cilnidipine and telmisartan in tablet and bulk dosage form by simultaneous equation and absorbance ratio method. International Journal of Pharmacy and Integrated Life Sciences 2014; 2(3): 59–74.

 

32. Kumar V D, Murugan S, Vetrichelvan T. Development and validation of first order derivative UV-spectrophotometric method for the estimation of metoprolol succinate, cilnidipine and telmisartan in bulk and tablet dosage form. World J Pharm Pharm Sci 2020; 9(12): 1257-1264.

 

33. Soni IJ, Panchal HJ. Development and validation of dual wavelength UV spectrophotometric method for simultaneous estimation of cilnidipine and olmesartan medoxomil in tablet dosage form. Indian Journal of Pharmaceutical and Biological Research 2014; 2(01): 76-81.

 

34. Ghelani N, Bhalodiya K, Faldu S, Dadhania K. Development and validation of spectrophotometric method for simultaneous estimation of olmesartan medoxomil and cilnidipine by simultaneous equation method. Pharma Tutor 2014; 2(6): 160-166.

 

35. Sidhdhapara M, Patel B, Parmar A, Vekariya H, Patel P. Development and validation of spectrophotometric method for simultaneous determination of cilnidipine and olmesartan medoximil in tablet dosage form. Int J Pharm Biol Sci 2014; (2): 97-101.

 

36. Sidhdhapara M, Patel B, Parmar A, Vekariya H, Patel P. Derivative Spectrophotometric Method for Simultaneous Determination of Cilnidipine and Olmesartan Medoximil in Tablet Dosage Form. Der Pharm Chem. 2014; 6: 175-178.

 

37. Ahmed M, Rashmi DR, Shetty AS, Anilkumar SM, Ravi MC, Kuppast IJ. RP-HPLC method development and validation for simultaneous estimation of cilnidipine and olmesartan medoxomil in combined tablet dosage form. World J Pharm Pharm Sci 2015; 4(1): 785-795.

 

38. Kalyankar GG, Patel J, Bodiwala KB, Lodha SR, Mistry V. Development and validation of first order UV derivative spectroscopy method for simultaneous estimation of cilnidipine and chlorthalidone in their combined tablet dosage form. Pharma Sci Monit 2019; 10(2): 101-111.

 

39. Patel SN, Hinge MA, Bhanushali VM. Development and validation of an UV spectrophotometric method for simultaneous determination of cilnidipine and chlorthalidone. J Pharm Res 2015; 9(1): 41-45.

 

40. Reddy BH, Spandana B, Mounika D, Devi S, Dacha A, Prakash KV. Simultaneous estimation of Telmisartan, Chlorthalidone and Cilnidipine by absorbance correction method using UV spectrophotometry. Indo Am J Pharm Res 2018; 5(3): 1998-2003.

 

41. Pandya H, Patel P B. Development and validation of analytical methods for the simultaneous estimation of metoprolol succinate, chlorthalidone and cilnidipine in tablet dosage form; World J Pharm Pharm Sci 2018; 7(5): 945-963.

 

42. Buchiya FV, Bhim AI, Raj HA, Jain VC. Simultaneous determination of cilnidipine and valsartan in synthetic mixture using spectrophotometric technique. Asian J of Pharma Analysis 2015; 5(1): 21-25. Doi-10.5958/2231-5675.2015.00004.6.

 

43. Jaldeepsinh VR, Maheshwari DG. Development and validation of second order derivative spectrophotometric method for simultaneous estimation of cilnidipine and valsartan in synthetic mixture. Ame J of Pharm Tech Res 2015; 2(5): 313-323.

 

44. Hetal VP, Tulsi A, Pinkal P. Development and Validation of First Order derivative UV Spectrophotometric Method for Simultaneous Estimation of Valsartan and Cilnidipine in Combination J. of Pharma. Res. 2015; 2319-5622.

 

45. Hinge MA, Desai DK, Patel ES, Singh R, Chavda R, Patel D. Development and validation of UV spectrophotometric method for simultaneous estimation of cilnidipine and metoprolol succinate in bulk drugs and combined dosage form. Der Pharmacia Lettre. 2015; 7: 299-306.

 

46. Agrawal O and Bansod T. Simultaneous estimation of cilnidipine and metoprolol succinate by uv-spectrophotometric and RP-HPLC method in bulk and solid dosage. Inventi Impact:Pharm Analysis and Quality Assurance 2016;4: 196-204.

 

47. Chaudhari VG, Vekaria HJ, Parmar AR. Development and validation of Q-absorbance ratio method for simultaneous estimation of cilnidipine and metoprolol succinate in their combined dosage form. Inventi Rapid: Pharm Analysis & Quality Assurance 2014; 2: 1.

 

48. Kadia TK, Shah DB, Dilip GM. Development and validation of Q-absorbance ratio spectrophotometric method for simultaneous estimation of cilnidipine and metoprolol succinate in bulk and combined dosage form. Int. J. Pharm. Pharm. Sci. 2014; 6(6): 401-407.

 

49. Jani RJ, Patel SA. Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Azilsartan Kamedoxomil and Cilnidipine in Synthetic Mixture. World J. Pharm. Res 2018; 7(8): 948-58.

 

50. Chaudhari SR, Shirkhedkar AA. An Investigative Review for Pharmaceutical Analysis of 1, 4-Dihydropyridine-3, 5-Dicarboxylic Acid Derivative: Cilnidipine. Critical reviews in analytical chemistry 2020; 29:1-0.

 

51. Patel H, Damahe DP, Narkhede SB. RP-HPLC Method Development and Validation for Simultaneous Estimation of Cilnidipine and Bisoprolol Fumarate in Tablet Dosage Form International Journal of Chem Tech Research 2019; 12(1): 269-276.

 

52. Thula KC, Patel DC, Maheshwari DG. Development and validation of first order derivative UV spectrophotometric method for simultaneous estimation of Nebivolol and Cilnidipine in pharmaceutical formulation. Int J Pharm Sci Rev Res 2015; 31(1): 243-247.

 

53. Patel PR, Patel N, Shah SK. Analytical Method Development and Validation for Simultaneous Estimation of Nebivolol Hydrochloride and Cilnidipine in Combined Dosage Form. J. Chem. Pharm. Res 2015; 7(9): 951-960.

 

54. El Hamd MA, Derayea SM, Abdelmageed OH, Askal HF. Spectrophotometric method for determination of five 1, 4-dihydropyridine drugs using N-bromosuccinimide and indigo carmine dye Int. J. Spectrosc. 2013; 243059: 1-7. doi.-10.1155/2013/243059

 

55. Chatpalliwar VA, Porwal PK, Upmanyu N. Validated gradient stability indicating HPLC method for determining Diltiazem Hydrochloride and related substances in bulk drug and novel tablet formulation. Journal of pharmaceutical analysis. 2012; 2(3): 226-37.

 

56. Nahar L, Onder A, Sarker SD. A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019). Phytochemical Analysis. 2020; 31(4): 413-457. Doi-10.1002/pca.2906

 

57. Kharat SS, Andhale SP, Saudagar RA. Validated RP-HPLC Method for Determination of Cilnidipine In Bulk And Pharmaceutical Dosage Form World J. Pharm. Pharm. Sci. 2017; 6(3): 1184-1195. Doi-10.20959/wjpps20173-8832

 

58. Buchiya FV, Raj HA, Jain VC, Bhatt M, Patel K. Method development and validation of RP-HPLC for simultaneous estimation of cilnidipine and valsartan in synthetic mixture. Int. J. Pharm. Chem. Anal. 2020; 7(3):119-124.

 

59. Kachave N, Kale M, Wagh D. Simultaneous estimation of Cilnidipine and Valsartan by RP-HPLC in Tablet Formulation. Eurasian Journal of Analytical Chemistry 2016; 11: 245-253.

 

60. Bhole RP, Pawara VC, Chitlange SS, Wankhede SB. Development and validation of HPLC method for simultaneous estimation of cilnidipine and valsartan in bulk and tablet dosage form. Res. Rev.: J. Pharm. Sci. 2015; 6(2):28-36.

 

61. Siddiqui MI, Srinivas M. Simultaneous estimation of Telmisartan and Cilnidipine in bulk and in tablet formulation using RP-HPLC. Pharmanest 2014; 5(3):2142-2148.

 

62. Pawar P, Gandhi SV, Deshpande PB, Padmanabh B, Vanjari S, Shelar SU. Simultaneous RP-HPLC estimation of Cilnidipine and Telmisartan in combined table dosage form. Chem. Sin. 2013; 4(2): 6-10.

 

63. Parihar Y, Kotkar T, Mahajan m, Sawant S. Development and validation of RP-HPLC method for simultaneous estimation of Telmisartan and Cilnidipine in bulk and tablet dosage form Pharmanest 2014; 5(5): 2321-2325.

 

64. Khandagale PY. RP-HPLC method development and validation for simultaneous estimation of Cilnidipine and Telmisartan in combined pharmaceutical dosage form. Int. Res. J. Pharm. 2017; 8(9): 118-121. Doi-10.7897/2230-8407.089166

 

65. Ahmed M, Rashmi R, Kuppast J. RP-HPLC method development and validation for simultaneous estimation of Cilnidipine and Olmesartan Medoxomil in combined tablet dosage form. World J. Pharm. Res 2014; 4: 785-795.

 

66. Amit SM, Manjusha ND, Sanjay DS. Development and validation of analytical method for simultaneous estimati on of Cilnidipine and Olmesartan medoxomil in bulk and tablet dosage form by RP-HPLC. Int J Pharm Pharm Sci. 2014; 6: 508–511.

 

67. Sidhdhapara MJ, Patel B, Paramar A. Development and validation of RP-HPLC method for simultaneous estimation of Cilnidipine and Olmesartan medoxomil in their combined tablet dosage form. Int J Pharm Biosci. 2014; 4(1):157-160.

 

68. Ghelani N, Dadhania K, Faldu S. Analytical method development and validation for simultaneous estimation of olmesartan medoxomil and cilnidipine in their combined pharmaceutical dosage form by RP-HPLC method. PharmaTutor. 2014; 2(7): 142-148.

 

69. Harshalatha P, Chandrasekhar KB Chandrasekhar MV. RP-HPLC method development and validation for the simultaneous estimation of antihypertensive drugs olmesartan and cilnidipine in bulk and tablet dosage form. Int J Pharm 2015; 5(4): 1248-1254.

 

70. Ravichandran S, Valliappan K, Ramanathan M. Development and Validation of Chromatographic Method for the Simultaneous Estimation of Olmesartan Medoxomil, Amlodipine Besylate, Cilnidipine in Combination Tablet Dosage Form. Int. J. Pharm. Pharm. Sci. 2015; 7:321-324

 

71. Patel RD, Luhar SV, Narkhede SB. Analytical Method Development and Validation for Simultaneous Estimation of Cilnidipine, Olmesartan and Chlorthalidone in Synthetic Mixture by RP-HPLC Method. J. Pharm. Sci. Bio-Sci. Res. 2016; 6: 308-314.

 

72. Shah P, Dhadhuk B. Related impurities high-performance liquid chromatography method development and validation for drug combinations: olmesartan medoxomil, chlorthalidone and cilnidipine. Int. J. Pharm. Sci. Drug Res. 2020;12(1): 1-10.

 

73. Nayak PR, Chaudhary AB, Rahevar NM. RP-HPLC method development and validation for simultaneous estimation of Chlorthalidone, Cilnidipine and Olmesartan in tablet dosage form. World J. Pharm. Res. 2016; 5(6): 1498-1508.

 

74. Vartak JP, Roy SMN. Simultaneous determination of Clinidipine, OLM and Chlorthalidone in pharmaceutical preparations using validated, LCMS compatible RP-HPLC method. Analytical Chemistry an Indian Journal. 2015; 15(3): 105-110.

 

75. Patel MP, Patel KP, Patel DB. Development and validation of analytical method for simultaneous estimation of cilnidipine, chlorthalidone and telmisartan in tablet dosage form. World J. Pharm. Pharm. Sci. 2016; 5(6): 1228-1241. Doi-10.20959/wjpps20166-6887

 

76. Kudumula N, Prasad YR. Development and validation of RP-HPLC Method for the Simultaneous Estimation of Chlorthalidone and Cilnidipine in Bulk and Combined tablet dosage form. Pharmacophore 2014; 5(4): 442-450.

 

77. Pawar VT, Pawar SV, More HN, Kulkarni AS, Gaikwad DT. RP-HPLC method for simultaneous estimation of cilnidipine and chlorthalidone. Res. J. Pharm. Technol. 2017; 10(11): 3990-3996. doi:10.5958/0974-360X.2017.00724.7

 

78. Bapnal M, Bhoi KG. Development and validation of analytical method for simultaneous estimation of cilnidipine and chlorthalidone in their combined dosage form. Int J Ayurveda Pharm Chem 2015; 2(3): 51-58.

 

79. Dagariya RK, Jat RK. Method development and validation of Irbesartan chlorthalidone and Cilnidipine in their combined tablet dosage form by high performance liquid chromatography. J. Drug Delivery Ther. 2017; 7(4):88-96.

 

80. Mourya ND, Prajapati Y, Sakhreliya B. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Chlorthalidone, Cilnidipine and Irbesartan in their Combined Marketed Dosage Form J Pharm Sci Bioscientific Res. 2017; 7(2):193-199.

 

81. Unnisa A. Analytical method development and validation for simultaneous estimation of cilnidipine and irbesartan in pharmaceutical dosage forms. World J. Pharm. Pharm. Sci. 2021; 10(3):1421-1428.

 

82. Solanki VS, Bishnoi RS, Baghel R, Jain D. RP-HPLC method development and validation for simultaneous estimation of Cilnidipine, Atenolol and Chlorthalidone. J. Drug Delivery Ther. 2018; 8(6): 78-82.

 

83. Pandya HB; Patel PB. Development and validation of analytical methods for the simultaneous estimation of metoprolol succinate, chlorthalidone and cilnidipine in tablet dosage form. World J. Pharm. Pharm. Sci. 2018; 7(5): 945-963.

 

84. Hinge MA, Desai DK, Patel ES. Simultaneous estimation of Cilnidipine and Metoprolol Succinate by RP-HPLC. Scholars Research Library. 2015; 7: 333-340.

 

85. Kadia TK, Shah DB, Dilip GM. Development and validation of q-absorbance ratio spectrophotometric method for simultaneous estimation of cilnidipine and metoprolol succinate in bulk and combined dosage form. Int. J. Pharm. Pharm. Sci. 2014; 6(6):401-7.

 

86. Varikuti PK, Lokeswara G, Haribaskar V, Gobinath M. Development and validation of RP-HPLC method for simultaneous estimation of metoprolol succinate and cilnidipine in combined tablet dosage form. Int J Pharm 2015; 5(4): 1196-1202.

 

87. Patel B, Chaudhary A, Parmar PJ, Patel VN. Development and validation of reversed phase high performance liquid chromatography method for simultaneous estimation of nebivolol hcl and cilnidipine In. Pharm. Biol. Eval. 2016; 3(2): 208-214.

 

88. Patel PR, Patel N, Shah SK. Analytical method development and validation for simultaneous estimation of nebivolol hydrochloride and cilnidipine in combined dosage form. J. Chem. Pharm. Res. 2015; 7(9): 951-960.

 

89. Patel H, Damahe PH, Luhar SV, Narkhede SB. Development and validation of uv spectrophotometric method for the simultaneous estimation of cilnidipine and bisoprolol fumarate in tablet dosage form. World J. Pharm. Pharm. Sci 2019; 7(11): 616-627. doi-10.20902/IJCTR. 2019.120130

 

90. Panda SS, Dutta S, Bera RK, Jammula S. Analytical eco-scale and quality by design-oriented liquid chromatography method for simultaneous quantification of metoprolol succinate, telmisartan, and cilnidipine in their fixed-dose combination. Separation Science Plus 2021; 4(3):128-136.

 

91. Khan A, Winter G, Tan S. Stability-indicating method development and validation for busulfan drug substance by gas chromatography-flame ionization detector. J of Chrom Sci 2021; 59(2):112-9.

 

92. Kalyankar TM, Wadher SJ, Bodhankar MR, Sayed MF. Stability Indicating Simultaneous Estimation of Phenylephrine HCl and Bromhexine HCl in Combined tablet dosage form by UV-Spectrophotometer. Res. J. Pharm. Technol. 2021; 14(6):3128-3132.

 

93. Tiwari B, Shirsat MK, Kulkarni A. Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Cilnidipine. J. Drug Delivery Ther. 2020; 10(1): 97-100.

 

94. Safhi M, Nagaraj MY. Development and validation of a Rapid Stability Indicating chromatographic determination of Cilnidipine in Bulk and Dosage form. Res. J. Pharm. Technol. 2013; 6(3):296-299.

 

95. Saravanan G, Praveen PN, Janeyulu IS, Visagaperumal D. Development and Validation of Stability Indicating RP-HPLC method for the simultaneous estimation of Metoprolol Succinate and Cilnidipine in bulk and pharmaceutical dosage form. Int. J. Pharm. Pharm. Sci. 2015; 7(1):150-154.

 

96. Shaikh SA, Pradhan PK, Upadhyay UM. Development and validation of analytical methods for estimation of cilnidipine and Metaprolol. International Journal of Pharma Sciences and Research 2015; 6(7):1067-1074.

 

97. Prasad KC, Krishna MR, Babu DJ, Kumar NA, Babu GR. Stablity-indicating simultaneous estimation of metoprolol and cilnidipine by using RP-HPLC. World J Pharm Pharm Sci 2016; 6(1):1395-1403.

 

98. Rupareliya RH, Joshi HS. Stability Indicating Simultaneous Validation of Telmisartan and Cilnidipine with Forced Degradation Behavior Study by RP-HPLC in Tablet Dosage Form. International Scholarly Research Notices. 2013; Article ID 461461. Doi-10.1155/2013/461461

 

99. Pooja JP, Ankit BC, Vijay PJ, Ruchi PS. Stability indicating RP-HPLC method development and validation for estimation of cilnidipine and valsartan. World J Pharm Pharm Sci. 2017; 5(5):1231-1245.

 

100. Patel ND, Mehta RS, Captain AD, Chavda AA. Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Cilnidipine and Nebivolol Hydrochloride in Tablet Dosage Form. Journalof pharmaceutical Science and Bioscientific Research, 2017; 7: 140-147

 

101. Sunitha N, Marihal SC, Sravanthi JS, Venu A, Rao BVN, Rao BA. Method development and validation of RP-HPLC method for the simultaneous estimation of Olmesartan and Cilnidipine in bulk and formulations. Int. J. Pharm. Res. Allied Sci. 2015; 4: 127-135.

 

102. Sawaikar L, Kapupara P. Development and Validation of a Stability indicating RP-HPLC Method for the Estimation of Chlorthalidone and Cilnidipine in Combined Pharmaceutical Dosage Form. J. Pharm. Technol. 2020; 13(5):2376-80.

 

103. Bommella M, Rao RN, Peddi P, Khagga1 M, Pal S. Development and Validation of a stability indicating RP-HPLC method for simultaneous determination of Telmisartan, Chlorthalidone and Cilnidipine in pharmaceutical combined dosage forms. Int J Pharm 2016; 6(2): 299-311.

 

104. Chinthala K, Krishnamurthy M, Kumar P. Stability indicating method development and validation for the simultaneous estimation of Olmesartan, chlortalidone and Cilnidipine in bulk and pharmaceutical dosage form by using RP-HPLC. International Journal of Pharmacy 2016; 6(3): 149-160.

 

105. Sharma A, Mishra A, Sharma S. Stability indicating simultaneous validation of telmisartan, cilnidipine and chlorthalidone with forced degradation behavior study by RP-HPLC in tablet dosage form. Int. J. Chem. Pharm. Sci. 2016; 7: 6-12.

 

106. Swapna G, Rahaman SA, Rani AP. Development and validation of stability indicating analytical method for simultaneous estimation of cilnidipine, chlorthalidone and telmisartan in bulk and tablet dosage form. Indian Drugs 2020; 57(5): 51-55.

 

107. Prajapati KM, Patel B, Patel J, Darji V, Jatinkumar D. Stability indicating RP-HPLC method development and validation for simultaneous estimation of cilnidipine and irbesartan in its pharmaceutical dosage form. World J Pharm Pharm Sci 2017; 6(5):1017-1026.

 

108. Patel DM, Parmar JN, Patel CN. Development and validation of stability indicating RP-HPLC method for Cilnidipine and Olmesartan medoxomil. International Journal of Pharmaceutical Research and Bio-Science. 2015; 4(3):69-82.

 

109. Rupareliya RH, Joshi HS, Khosla E. Stability Indicating Simultaneous Validation of Telmisartan and Cilnidipine with Forced Degradation Behaviour Study by RP-UPLC in Tablet Dosage Form. International Journal of Pharmaceutical Quality Assurance 2016; 7: 39-45.

 

110. Ch KR, JV SK, Palusa SK. Isolation and characterization of novel degradation products in cilnidipine by LC-QTOF-MS/MS, LCMS n, 2D-NMR and FTIR. New Journal of Chemistry 2018; 42(1):634-46.

 

111. Deshmukh R, Sharma L, Tekade M, Kesharwani P, Trivedi P, Tekade RK. Force degradation behavior of glucocorticoid deflazacort by UPLC: isolation, identification and characterization of degradant by FTIR, NMR and mass analysis. J. biomed. res. 2016; 30(2):149.

 

112. Alagar RM. A New Analytical Method Development and Validation for the Estimation of Olmesartan Medoxomil and Cilnidipine in Its Pharmaceutical Dosage Form by UPLC as per ICH Guide lines, Asian Journal of Research in Biological and Pharmaceutical Sciences, 2015; 3(2): 78 – 86.

 

113. Guideline ICH. Validation of analytical procedures: text and methodology. Q2 (R1). 2005; 1(20):05.

 

114. Chatki PK, Tabassum S. Analytical Methods of Dihydropyridines Based Calcium Channel Blockers-Amlodipine, Lacidipine, Isradipine, Nifedipine, Felodipine, Cilnidipine and its related formulations: A Review. Asian Journal of Research in Chemistry 2021; 14(3):221-234.

 

115. Lee KR, Chae YJ, Lee JH, Kim DD, Chong S, Shim CK, Chung SJ. Quantification of cilnidipine in human plasma by liquid chromatography-mass spectrometry. Journal of liquid chromatography & related technologies. 2012; 35(2): 308-320.

 

116. Lee HW, Seo JH, Lee HS, Jeong SY, Cho YW, Lee KT. Development of a liquid chromatography/negative-ion electrospray tandem mass spectrometry assay for the determination of cilnidipine in human plasma and its application to a bioequivalence study. J. Chromatogr. B. 2008; 862(1-2): 246-251.

 

117. Muralidharan S, Kumar J R, Dhanaraj SA. Simple and Effective HPLC Method Development and Its Validation for Clindipine in Human Drug Free Plasma. Pak. J. Pharm. Sci. 2015; 28: 135–138.

 

118. Zhang X, Zhai S, Zhao R, Ouyang J, Li X, Baeyens WR. Determination of cilnidipine, a new calcium antagonist, in human plasma using high performance liquid chromatography with tandem mass spectrometric detection. Analytica chimica acta. 2007; 600(1-2):142-146.

 

119. Kachave RN, Yelmame SS, Mundhe AG. Quantitative estimation of cilnidipine and valsartan in rat lasma by RP-HPLC: its pharmacokinetic application. Future Journal of Pharmaceutical Sciences. 2021; 7(1):1-7.

 

120. Eswarudu MM, Rao AL, Vijay K. Bioanalytical method development and validation for simultaneous determination of chlorthalidone and cilnidipine drugs in human plasma by RP-HPLC. International journal of research in pharmacy and chemistry 2019; 9(1), 33-44.

 

121. Aruna G, Bharathi K, Kvsrg Prasad. Development and validation of bioanalytical HPLC method for simultaneous estimation of cilnidipine and nebivolol in human plasma. Int J Pharm Pharm Sci 2017; 9(10):253-259.

 

122. Shah DM, Doshi DB. Development and validation of HPTLC method for simultaneous estimation of nebivolol hydrochloride and cilnidipine in combined pharmaceutical tablet dosage form. Int J Pharma Res Rev 2016;5(6):1-7.

 

123. Karmalkar HS, Vaidya VV, Gomes NA, Choukekar MP, Kekare MB. Determination of cilnidipine from pharmaceutical formulation by high performance thin layer chromatographic method. Analytical chemistry. 2008; 7(8):573-576.

 

124. Minase AS, Dole MN. Development and Validation of Analytical Method for Simultaneous Estimation of Cilnidipine and Olmesartan Medoxomil in Bulk and Tablet Dosage form by HPTLC. Journal of Advanced scientific Research, 2014; 5: 34-38.

 

125. Dedhiya PP, Patel CJ, Chauhan RS, Kalyankar Gg, Vyas Rh, Shah Sa. Development and validation of HPTLC method for simultaneous estimation of Olmesartan medoxomil and Cilnidipine in their combined pharmaceutical dosage forms. Journal of Pharmacy and Applied Sciences 2015; 2(2):21-27.

 

126. Soni V, Kakadiya J, Patel P, Shah N. Devlopment and Validation of High Performance Thin Layer Chromatographic method for Cilnidipine and Metoprolol Succinate in their combined pharmaceutical dosage form. Int J Pharma Nano Sci. 2014; 61: 61–72.

 

127. Desai D, Vashi N, Dalvadi H, Desai S, Hinge M. HPTLC Method Development and Validation of Cilnidipine and Metoprolol Succinate in Combined Dosage Form. Pharma Methods. 2016; 7(1): 28-34. doi- : 10.5530/phm.2016.7.5

 

128. Pawar P, Deshpande P, Gandhi S, Bhavani V. High Performance Thin Layer Chromatographic deermination of Cilnidipine and Telmisartan in combined tablet dosage form. International Research Journal of Pharmacy. 2012; 3(6):219-222.

 

129. Butle SR, Deshpande PB. Development and validation of stability- Indicating HPTLC method for simultaneous determination of Telmisartan and Cilnidipine in combined tablet dosage form. International Journal of Pharmaceutical Sciences and Drug Research. 2015; 7(6): 478-483.

 

130. Bhole RP, Pawara VC, Chitlange SS, Wankhede SB. Development and validation of HPTLC method for simultaneous estimation of cilnidipine and valsartan in bulk and tablet dosage form. In Conference on Harmonization (ICH) guidelines 2015; Vol. 14, 21.

 

131. Rathod RH, Patil AS, Shirkhedkar AA. Novel NP and RP-HPTLC in Praxis for Simultaneous Estimation of Chlorthalidone and Cilnidipine in Bulk and Pharmaceutical Formulation. Analytical Chemistry Letters. 2018; 8(6):862-71. doi-10.1080/22297928.2018.1527252

 

132. Ghante MR, Akhade N, Gota P, Nikam A, Jagtap S, Nikam V. Development and validation of high-performance thin-layer chromatography method for simultaneous estimation of Nebivolol hydrochloride and Cilnidipine. Development. 2019; 12(4).

 

133. Salunkhe MN, Gite SD, Kachave RN. Recent trends in impurity profiling and forced degradation of antihypertensive drugs. J. Liq. Chromatogr. 2017; 40(16):813-831. doi-10.1080/10826076.2017.1373670.

 

134. Dhangar KR, Jagtap RB, Surana SJ, Shirkhedkar AA. Impurity profiling of drugs towards safety and efficacy: theory and practice. J. Chilean Chem Soc. 2017; 62(2):3543-3557. doi-10.4067/S0717-97072017000200024.

 

135. Kasimala BB, Anna VR, Mallu UR. Article Details Stability-indicating reversed-phase HPLC method for the separation and estimation of related impurities of Cilnidipine in pharmaceutical formulations. 2018; 55 (12):41-49.


136. Masada S, Tsuji G, Arai R, Uchiyama N, Demizu Y, Tsutsumi T, Abe Y, Akiyama H, Hakamatsuka T, Izutsu KI, Goda Y. Rapid and efficient high-performance liquid chromatography analysis of N-nitrosodimethylamine impurity in valsartan drug substance and its products. Scientific reports. 2019;9(1): 1-6.

 

137. Zeng H, Wang F, Zhu B, Zhong W, Shan W, Wang J. Study of the structures of photodegradation impurities and pathways of photodegradation of cilnidipine by liquid chromatography/Q‐Orbitrap mass spectrometry. Rapid Communications in Mass Spectrometry. 2016; 30(15):1771-8.

 

138. Hu CC, Gu X. Structural analysis of light degradation impurity of cilnidipine. Chin. J. Pharm. Anal. 2016; 36(8):1446-1450.

 

139. Wang SG, Gu P. Determination of cilnidipine related substances by RP-HPLC [J]. Anhui Medical and Pharmaceutical Journal. 2011 10.

 

140. Zeng H, Wang J, Zhu B, Shao P, Zhong W. Rationality Evaluation of Packaging Materials and Study on Impurity Profiling of Cilnidipine Preparations by HPLC-Q-TOF/MS. Current Pharmaceutical Analysis. 2020;16(2):220-30.

 

141. Du Y, Di B, Chen J, Zheng Z. Development of desulfated chondroitin sulfate C as a novel chiral selector in capillary electrophoresis and enantioseparation of cilnidipine. Chinese journal of chromatography. 2004; 22(4):382-385.

 

142. Zhang L, Song Z, Dong Y, Wang Y, Li X, Long H, Xu K, Deng C, Meng M, Yin Y, Xi R. Enantiomeric separation of 1, 4-dihydropyridines by liquid-phase microextraction with supercritical fluid chromatography. The Journal of Supercritical Fluids. 2016; 107:129-136.

 

143. Rahman A, Sravani GJ, Srividya K, Priyadharshni AD, Narmada A, Sahithi K, Sai TK, Padmavathi Y. Development and Validation of Chemometric Assisted FTIR Spectroscopic Method for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Pure and Pharmaceutical Dosage Forms. Journal of Young Pharmacists. 2020; 12(2):S51.

 

144. Patel A, Panchal A, Patel V, Nagar A. FTIR spectroscopic method for quantitative analysis of Cilnidipine in tablet dosage form. International Journal of Pharma Sciences and Research. 2015; 4(6).

 

145. Jain R. An electrochemical sensor based on synergistic effect of nano zinc oxide-multiwalled carbon nanotubes hybrid film for sensing of calcium antagonist cilnidipine. Journal of the Electrochemical Society. 2013; 160(10):H645.

 

146. Tan S, Jiang J, Shen G, Shen G, Yu R. A novel fluorescence probe for cilnidipine assay. Analytica chimica acta; 2005; 547(2):215-220. Doi- 10.1016/j.aca.2005.05.041.

 

Author Information


Pranali Mishra

Email - pranaliankit@gmail.com

Affiliation of the corresponding author-

School of Pharmacy,

L N University,

Kolar Road,

Bhopal-462042

Madhya Pradesh.

India

Mobile no.-+919425677620

ORCID No - 0000-0002-6040-5232


Email of other authors:


Ankit Mishra

mishraaa@gmail.com

Department of Pharmaceutics,

Faculty of Pharmacy,

VNS Group of Institutions,

Neelbud, Bhopal, MP, India

Pin-462044

mobile no.-+919827930533

ORCID No – 0000-0003-0795-8417


Parul D Mehta

parulmehta1@rediffmail.com

Mobile no.-+918602718148





コメント


bottom of page